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CSF2 (Human) Recombinant ProteinBioActive

  • Catalog # : P4395
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  • Specification
  • Product Description:
  • Human CSF2 (P04141) recombinant protein expressed in Escherichia coli.
  • Sequence:
  • MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 14.5
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by the dose-dependent proliveration of human TF-1 cells. The expected ED50 for this effect is 0.14-0.2 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue

    QC Testing of P4395
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • Lyophilized from 10 mM Na2PO4, pH 7.5
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Serial dilutions of human CSF2 (starting at 25 ng/mL) were added to TF1 cells. After 66 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1437
  • Gene Name:
  • CSF2
  • Gene Alias:
  • GMCSF,MGC131935,MGC138897
  • Gene Description:
  • colony stimulating factor 2 (granulocyte-macrophage)
  • Gene Summary:
  • The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq
  • Other Designations:
  • colony stimulating factor 2,granulocyte-macrophage colony stimulating factor,molgramostin,sargramostim
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