CSF2 (Human) Recombinant Protein
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Specification
Product Description
Human TNF (127 a.a.) full-lenth recombinant protein expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host
Plants
Theoretical MW (kDa)
30, 38
Reactivity
Human
Form
Lyophilized
Preparation Method
Hordeum vulgare (barley) expression system
Purification
Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by LAL assay.
Activity
Activity has been measured by cell-based proliferation assay using TF-1 cells. ED50 is 0.05 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of CSF2 (Human) Recombinant Protein (Cat # P5885) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — CSF2
Entrez GeneID
1437Gene Name
CSF2
Gene Alias
GMCSF, MGC131935, MGC138897
Gene Description
colony stimulating factor 2 (granulocyte-macrophage)
Omim ID
138960Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq
Other Designations
colony stimulating factor 2|granulocyte-macrophage colony stimulating factor|molgramostin|sargramostim
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