ARHGDIA monoclonal antibody (M02), clone 1G5-2F3
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ARHGDIA.
Immunogen
ARHGDIA (AAH16031, 1 a.a. ~ 204 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.18 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ARHGDIA monoclonal antibody (M02), clone 1G5-2F3. Western Blot analysis of ARHGDIA expression in human ovarian cancer.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGDIA is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — ARHGDIA
Entrez GeneID
396GeneBank Accession#
BC016031Protein Accession#
AAH16031Gene Name
ARHGDIA
Gene Alias
GDIA1, MGC117248, RHOGDI, RHOGDI-1
Gene Description
Rho GDP dissociation inhibitor (GDI) alpha
Omim ID
601925Gene Ontology
HyperlinkGene Summary
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Comparative proteomic analysis on human L-02 liver cells treated with varying concentrations of trichloroethylene.
Liu J, Huang H, Xing X, Xi R, Zhuang Z, Yuan J, Yang F, Zhao J.
Toxicology and Industrial Health 2007 Mar; 23(2):91.
Application:WB-Ce, Human, L-02 liver cells.
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Comparative proteomic analysis on human L-02 liver cells treated with varying concentrations of trichloroethylene.
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