Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product:
DAPI Counterstain (1500 ng/mL ) 250 uL
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome:
Probe 1: Size: Fluorophore: Location:
CDH1 Approximately 390kb Texas Red 16q22.1
Probe 2: Size: Fluorophore: Location:
CEN16q Approximately 700kb FITC 16q12.1
Probe Gap:
The gap between two probes is approximately 19,100 kb.
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq