Cd34 monoclonal antibody, clone MEC 14.7
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100).
Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles.
(A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells.
(B) Cd34 expression in a murine telogen hair follicle.
(C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C.
Scale bars: 100 mum.
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Specification
Product Description
Rat monoclonal antibody raised against Cd34.
Immunogen
Full length mouse Cd34.
Host
Rat
Reactivity
Mouse
Specificity
The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries.
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a
Recommend Usage
Western Blot (1:250)
Flow Cytometry (1 ug per million cells)
Immunocytochemistry/Immunofluorescence (1:100-1:1000)
Immunohistochemistry (1:250)
Immunoprecipitation (1:10-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100).
Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles.
(A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells.
(B) Cd34 expression in a murine telogen hair follicle.
(C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C.
Scale bars: 100 mum.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — Cd34
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Publication Reference
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Preclinical studies on the use of a P-selectin-blocking monoclonal antibody to halt progression of myelofibrosis in the Gata1low mouse model.
Paola Verachi, Francesca Gobbo, Fabrizio Martelli, Mario Falchi, Antonio di Virgilio, Giuseppe Sarli, Celine Wilke, Andreas Bruederle, Anirudh Prahallad, Francesca Arciprete, Maria Zingariello, Anna Rita Migliaccio.
Experimental Hematology 2023 Jan; 117:43.
Application：IHC, Mouse, Mouse bone marrow, Mouse spleen.
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Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW.
The Journal of Investigative Dermatology 2003 Apr; 120(4):501.
Application：IF, IHC-P, Flow Cyt, Mouse, Skin, keratinocytes.
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Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
Garlanda C, Berthier R, Garin J, Stoppacciaro A, Ruco L, Vittet D, Gulino D, Matteucci C, Mantovani A, Vecchi A, Dejana E.
Eur J Cell Biol 1997 Aug; 73(4):368.
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Preclinical studies on the use of a P-selectin-blocking monoclonal antibody to halt progression of myelofibrosis in the Gata1low mouse model.
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