CDH2 monoclonal antibody, clone 5D5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) against A-431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of A-431 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of PC-2 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CDH2.
Immunogen
Recombinant protein corresponding to human CDH2.
Host
Mouse
Theoretical MW (kDa)
99.8
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) against A-431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) with DAB staining.Immunofluorescence
Immunofluorescence analysis of A-431 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-2 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green) and negative control (purple). -
Gene Info — CDH2
Entrez GeneID
1000Gene Name
CDH2
Gene Alias
CD325, CDHN, CDw325, NCAD
Gene Description
cadherin 2, type 1, N-cadherin (neuronal)
Omim ID
114020Gene Ontology
HyperlinkGene Summary
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq
Other Designations
N-cadherin 1|cadherin 2, N-cadherin (neuronal)|cadherin 2, type 1|calcium-dependent adhesion protein, neuronal|neural cadherin
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Interactome
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Pathway
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Disease
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