BID monoclonal antibody, clone 3C5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) against HeLa (1), A-431 (2), Jurkat (3), A-549 (4), HepG2 (5), and HEK293 (6) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and tonsil tissues (B) using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with BID monoclonal antibody, clone 3C5 (Cat # MAB10503).
Flow Cytometry
Flow cytometric analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BID.
Immunogen
Recombinant protein corresponding to human BID.
Host
Mouse
Theoretical MW (kDa)
22
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) against HeLa (1), A-431 (2), Jurkat (3), A-549 (4), HepG2 (5), and HEK293 (6) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and tonsil tissues (B) using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with BID monoclonal antibody, clone 3C5 (Cat # MAB10503).Flow Cytometry
Flow cytometric analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green) and negative control (purple). -
Gene Info — BID
Entrez GeneID
637Gene Name
BID
Gene Alias
FP497, MGC15319, MGC42355
Gene Description
BH3 interacting domain death agonist
Omim ID
601997Gene Ontology
HyperlinkGene Summary
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
BH3-interacting domain death agonist|BID isoform ES(1b)|BID isoform L(2)|BID isoform Si6|Human BID coding sequence|OTTHUMP00000196197|apoptic death agonist|desmocollin type 4
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Interactome
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Pathway
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Disease
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