Systems & Automations

Description

Spiraltor™ 48 - Spiral-Mix Magnetic Bead Handling is a patented spiral-mix, magnetic bead handling system for high throughput IVT mRNA and circRNA purification. Combining 48 channel open platform with oligo (dT) magnetic beads, Spiraltor™ 48 easily handles 48 different assays simultaneously by transferring beads from one well to the next for spiral-mixing, binding, washing, and elution reactions via the robotic action of parallel magnetic rods. Spiraltor™ 48 simplifies the routine yet labor-intensive process and addresses the needs of rigorous mRNA applications such as protein replacement, mRNA antibody therapy, personalized cancer vaccines, and infectious mRNA vaccines. With oligo (dT) magnetic beads separation method, there’s no need for centrifugation or vacuum, eliminating stress or shear force and delivering pure, intact mRNA and circRNA. Spiraltor™ 48 delivers reproducible and consistent results by obviating the drawbacks of manual operation. Its intuitive user interface allows easy change of parameters tailored to your experiment.

Description

Abnova’s Precipitor™ 32 Plus system is an automated magnetic bead platform for high throughput extraction, purification, and precipitation. Combining 96 deep well plate with conjugated magnetic beads, Precipitor™ 32 Plus easily handles 32 different assays simultaneously by transferring beads from one well to the next for mixing, binding, washing, and elution reactions via the robotic action of parallel magnetic rods. Precipitor™ 32 Plus simplifies the routine yet labor-intensive process and addresses the needs of rigorous proteomic screening and biomarker discovery applications such as nucleic acid extraction, recombinant protein purification, immunoprecipitation (IP, ChIP, RIP), and protein-protein interaction. Precipitor™ 32 Plus delivers reproducible and consistent results by obviating the drawbacks of manual operation. Its integrated on-screen display allows easy change of parameters tailored to your experiment.

Description

Abnova’s LiquidCell™ is a non-invasive, negative enrichment system for depletion of white blood cells (WBCs) and enumeration and retrieval of circulating rare cells (CRCs). Three major subtypes of CRCs in translational research and clinical studies are circulating cells (CTCs), circulating progenitor cells (CPCs), and circulating fetal cells (CFCs). A challenge for market adoption of CRCs is the efficient and reproducible identification, single cell isolation and retrieval of highly pure and viable CRCs, with their applications supported by a wide repertoire of standardized bioreagents. LiquidCell™ platform utilizes AbBeads™, SuperSlide™, tyramide TYRx™ and chromogenic CHRx™ technologies to enable CRC isolation and detection. AbBeads™ are antibody functionalized magnetic beads for white blood cell (WBC) depletion while avoiding the concomitant loss of CRCs. SuperSlide™ is a specialty-coated, non-autofluorescent glass slide for maximal CRC capture and retention throughout the cell processing. TYRx™ utilizes tyramide fluorescence and horseradish peroxidase (HRP)-conjugated secondary antibody for signal enhancement of low-density proteins on CRCs. CHRx™ uses DAB and Fast Red chromogenic substrates combined with horseradish peroxidase (HRP) and alkaline phosphatase (AP) to deliver brown and red color staining of CRCs under visible light. Monoclonal antibodies targeting the intracellular and cell surface proteins can be flexibly interchanged to accommodate the biomarker switch in disease progression. This results in highly pure and viable CRCs for downstream protein characterizations, gene analysis, and cell assays. CTC enumeration is currently the only FDA approved application as a prognostic marker in metastatic breast, prostate, and colorectal cancers. In contrast, effective single cell isolation and retrieval of CRCs will open up new clinical utilities in the diagnostic and pharmaceutical arena.

Description

CytoView™ is an integrated all-in-one imaging system with artificial intelligence (AI) solution for the identification of circulating tumor cells and circulating fetal cells. Cell-based liquid biopsy is a multi-step, diagnostic process requiring blood sample preparation, cell enrichment, immunostaining, and molecular analysis such as fluorescence in situ hybridization (FISH) and next-generation sequencing (NGS). The immunostaining and FISH analysis is a critical component of the cell distinction requiring high-speed cell scanning with smart artificial intelligence (AI) coupled with direct sample viewing to accelerate and shorten the cycle time from blood sample to report. Most scanning microscopes in the market do not have AI capability to analyze the captured images. Moreover, the scanning microscope does not always have sufficient viewing resolution for final confirmation. CytoView™ distinguishes each cell’s protein and genomic signature, and more importantly their intercellular heterogeneity and homogeneity. This foremost identification is first validation step followed by more intensive DNA and RNA validation at greater breadth and depth. CytoView™ is a highly competitive, imaging and smart-algorithm platform customized for the non-invasive, cell-based liquid biopsy market.

Description

Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs). Three major subtypes of CRCs in translational research and clinical studies are circulating tumor cells (CTCs), circulating progenitor cells (CPCs), and circulating fetal cells (CFCs). A challenge for market adoption of CRCs is the efficient and reproducible identification, single cell isolation, and retrieval of highly pure and viable CRCs, with their applications supported by a wide repertoire of standardized grade bioreagents. CytoQuest™ CR technology utilizes SCx™ spiral chamber, HBx™ micromixer, antibody immobilized nanoarray, and TCx™ thermal control to enable a multitude of CRC functions. SCx™ spiral chamber is equipped with a non-sticky coil and a self-contained micro-vibrator for unimpeded delivery of pretreated blood sample into the nanoarray. HBx™ micromixer provides a herringbone conduit for cell mixing. CytoChipNano is a streptavidin nanoarray which captures the CRCs for cell enumeration and single cell isolation by laser microdissection or micromanipulation. CytoChipNano CR is a specialized streptavidin nanoarray with thermo-sensitive coating which captures and releases the CRCs via a TCx™ thermal control of alternating temperatures. Monoclonal antibodies targeting specific surface biomarkers of circulating rare cells can be flexibly interchanged to accommodate the biomarker switch in CRC progression. This results in highly pure and viable CRCs for downstream protein characterizations, gene analysis, and cell assays. Cell enumeration is currently the only FDA approved application as a prognostic marker in breast, prostate, and colorectal cancers. In contrast, effective single cell isolation and retrieval of CRCs opens up new scope of applications in the diagnostic and pharmaceutical industry.

Description

High Throughput Biomarker Discovery and Validation. Tissue microarray is a powerful, high throughput platform for prognostic, diagnostic, and therapeutic target discovery and validation. This technology was developed by Kononen and colleagues in 1998 and has become an integral part of the translational research tool in protein profiling in the Proteomic era. The arrays are generated from the core needle biopsy of the paraffin-embedded tissue blocks and juxtaposed in a high density format on a glass slide for comparative, protein expression profiling for statistical analysis. As such, a large number of tissue specimens from a broad spectrum of diseases and clinical parameters can be screened against their counterparts to identify and validate biomarkers which have significant impact on disease management and treatment. Antibody plays a pivotal role in the characterization of protein expression in the tissue microarray. Notably, the availability of a large collection of validated antibodies is crucial to the successful screening and validation process. Abnova has opened up this bottleneck by building the largest antibody library for immunohistochemistry, tissue microarray and clinical applications.

Description

Abnova’s SpiralPipet™ is a patented spiral-mix, magnetic bead handling system for high throughput precipitation, purification, and extraction. Combining 96 deep well plate with affinity conjugated magnetic beads, SpiralPipet™ easily handles 8 different assays simultaneously by transferring beads from one well to the next for spiral-mixing, binding, washing, and elution reactions via the robotic action of parallel magnetic rods. SpiralPipet™ can also work independently as a stand-alone pipet, allowing greater freedom to expedite your work. SpiralPipet™ simplifies the routine yet labor-intensive process and addresses the needs of rigorous proteomic screening and biomarker discovery applications such as immunoprecipitation(IP, ChIP, RIP), recombinant protein purification, protein-protein interaction, and nucleic acid extraction. SpiralPipet™ delivers reproducible and consistent results by obviating the drawbacks of manual operation. Its integrated on-screen, pipet display allows easy change of parameters tailored to your experiment.