Synthetic circular RNA (circRNA) sponge acts as a regulatory molecule tool in the complex network of gene expression. It functions by sequestering and binding specific microRNAs (miRNAs), acting as competitive inhibitors. Compared to linear RNAs, circRNA sponges are more resistant to exonucleolytic degradation. circRNA sponges overcome the toxicity exhibited by anti-miRNA oligonucleotides (AMO) and dosage constraint by the plasmid-based miRNA sponge. Additionally, circRNA sponges do not necessitate nucleoside modifications and possess inherent non-immunogenic properties. Abnova offers you premium custom circRNA sponges. Contact us today to discuss your project and embark on a journey of precision gene regulation together!

Advantages
  • Enhanced stability
  • Efficient miRNA sequestration
  • Long-lasting regulatory effects
  • Precise gene expression control
 
 

Applications
  • Gene regulation engineering
  • Functional studies of miRNAs
  • Biomarker discovery and diagnostics
  • Therapeutic interventions
 
 

Flowchart
circRNA-Sponges-flow.png (32 KB)
 
 
 

Examples

miR-122-5p circRNA sponge absorbs miR-122 and derepress TGFβR1 transcription in mouse cells
 
circRNA-data.png (4 KB)
 
Western blot analysis of TGFβ receptor 1 (TGFβR1) in Hepa1-6 cells with treated artificial circRNA sponge for miR-122 (122sp) and untreated negative control (NC). The silencing of miR-122 by circRNA sponge in Hepa1-6 increases TGFβR1 expression.
 
 

miR-122-5p circRNA sponge affinity pulldown assay
 
circRNA-data2.png (15 KB)
 
E-Gel™ EX 2% Agarose Gel of circRNA pulldown.
Lane 1: The binding complex, miR-122-5p circRNA sponge 313nt, and biotinylated miR-122-5p was pulled down by Streptavidin Magnetic Microspheres.
Lane 2: miR-122-5p circRNA sponge alone not pulled down by Streptavidin Magnetic Microspheres.
 
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