CD74 recombinant monoclonal antibody, clone LL1 (EPB1)
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD74.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against whole Raji cells or a crude membrane preparation.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Radioimmunoassay
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot anaysis of Lane 1: human spleen lysate and Lane 2: human tonsil lysate with CD74 recombinant monoclonal antibody, clone LL1 (EPB1) (Cat # RAB03330).Immunofluorescence
Immunofluorescent staining of Daudi cells with CD74 recombinant monoclonal antibody, clone LL1 (EPB1) (Cat # RAB03330).
Daudi cells on Shi-fix™ cover-slips stained with the chimeric r version of RAB03330 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03330- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Radioimmunoassay
Flow Cytometry
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Gene Info — CD74
Entrez GeneID
972Gene Name
CD74
Gene Alias
DHLAG, HLADG, Ia-GAMMA
Gene Description
CD74 molecule, major histocompatibility complex, class II invariant chain
Omim ID
142790Gene Ontology
HyperlinkGene Summary
major histocompatibility complex
Other Designations
CD74 antigen|CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II antigens
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Interactome
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Pathway
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Disease
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