AKT1 recombinant monoclonal antibody, clone AktT308-G12
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More Files
- More Functions
Western Blot
Western blot analysis of 293T cell extract untreated or treated with 20% FBS using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.1 ug/mL.
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells treated with LY294002+U0126+wortmanin and stained with secondary antibody as negative control (blue) or treated with LY294002+U0126+wortmain (red) or with PDGF (green) and stained using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.01 ug/mL.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AKT1.
Antibody Species
Rabbit
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr308 of human phospho Akt1
Reactivity
Human
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 293T cell extract untreated or treated with 20% FBS using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.1 ug/mL.Flow Cytometry
Flow cytometric analysis of NIH3T3 cells treated with LY294002+U0126+wortmanin and stained with secondary antibody as negative control (blue) or treated with LY294002+U0126+wortmain (red) or with PDGF (green) and stained using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.01 ug/mL. -
Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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