ADSS2 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with ADSS recombinant monoclonal antibody (Cat # RAB02464).
Immunofluorescence
Immunofluorescent analysis of HeLa cells with ADSS recombinant monoclonal antibody (Cat # RAB02464). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ADSS2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human AdSS 2.
Theoretical MW (kDa)
54
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of ADSS protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with ADSS recombinant monoclonal antibody (Cat # RAB02464).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with ADSS recombinant monoclonal antibody (Cat # RAB02464). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — ADSS
Entrez GeneID
159Protein Accession#
P30520Gene Name
ADSS
Gene Alias
ADEH, MGC20404
Gene Description
adenylosuccinate synthase
Omim ID
103060Gene Ontology
HyperlinkGene Summary
Adenylosuccinate synthetase catalyzes the first committed step in the conversion of IMP to AMP [provided by RefSeq
Other Designations
Adenylosuccinate synthetase (Ade(-)H-complementing)|OTTHUMP00000037982
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Interactome
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Pathway
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Disease
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