ENO1 recombinant monoclonal antibody, clone R01-4C8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ENO1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human ENO1.
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ENO1 in Jurkat, rat Brain, C6, Hela lysates using ENO1 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — ENO1
Entrez GeneID
2023Protein Accession#
P06733Gene Name
ENO1
Gene Alias
ENO1L1, MBP-1, MPB1, NNE, PPH
Gene Description
enolase 1, (alpha)
Omim ID
172430Gene Ontology
HyperlinkGene Summary
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|OTTHUMP00000001706|alpha enolase like 1|enolase 1|non-neural enolase|phosphopyruvate hydratase|tau-crystallin
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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