SARS-CoV/SARS-CoV-2 N IgG1 recombinant human antibody
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Specification
Product Description
Human recombinant monoclonal antibody raised against SARS-CoV/SARS-CoV-2 N.
Antibody Species
Human
Source / Expression System
293
Reactivity
SARS-CoV, SARS-CoV-2
Specificity
This antibody recognizes human SARS Coronavirus Nucleocapsid protein.
Form
Liquid
Purification
Protein A purification
Purity
> 98% (SDS-PAGE)
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Proteins.
Western Blot analysis of different recombinant proteins were stained with this antibody at 1:1000 dilution. Lane 1: SARS-CoV-2 NP, Lane 2: SARS-CoV NP, Lane 3: MERS-CoV NP and Lane 4: HCoV-NL63 NP.
Recommend Usage
ELISA (1:5000-1:20000)
Flow Cytometry (1:500-1:1000)
Immunofluorescence(1:500-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at 4°C for at least 6 months. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Multi-cycle kinetic analysis
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 cells and Lane 2: transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with this antibody at 1:1000 dilution.Western Blot (Recombinant protein)
Immunofluorescence
Immunofluorescent staining of 293T cells were respectively transfected with SARS-CoV, SARS-CoV-2, MERS-CoV and HCoV-NL63 nucleocapsid plasmid and stained with this antibody (1:500).Enzyme-linked Immunoabsorbent Assay
ELISA assay of this antibody to SARS-CoV-2 nucleocapsid protein, SARS-CoV nucleocapsid protein, MERS-CoV nucleocapsid protein, HCoV-NL63 nucleocapsid protein and BSA (BSA: negative control).Flow Cytometry
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