TRIM28 polyclonal antibody
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More Files
- More Functions
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM28.
Immunogen
A synthetic peptide corresponding to C-terminus of human TRIM28.
Sequence
C-SSQELSGGPGDGP
Host
Goat
Theoretical MW (kDa)
92
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
Immunofluorescence (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM28 polyclonal antibody (Cat # PAB6320) (4 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM28
Entrez GeneID
10155Protein Accession#
NP_005753.1Gene Name
TRIM28
Gene Alias
FLJ29029, KAP1, RNF96, TF1B, TIF1B
Gene Description
tripartite motif-containing 28
Omim ID
601742Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq
Other Designations
KRAB-associated protein 1|nuclear corepressor KAP-1|transcriptional intermediary factor 1-beta|tripartite motif-containing 28 protein
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Interactome
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Publication Reference
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KAP1 Is Associated With Peritoneal Carcinomatosis in Gastric Cancer.
Yokoe T, Toiyama Y, Okugawa Y, Tanaka K, Ohi M, Inoue Y, Mohri Y, Miki C, Kusunoki M.
Annals of Surgical Oncology 2010 Mar; 17(3):821.
Application:IHC-P, Human, Gastric cancer.
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KAP1 Is Associated With Peritoneal Carcinomatosis in Gastric Cancer.
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