WISP1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WISP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human WISP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate (35 ug/lane) with WISP1 polyclonal antibody (Cat # PAB4835).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WISP1 polyclonal antibody (Cat # PAB4835) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WISP1
Entrez GeneID
8840Protein Accession#
WISP1_HUMANGene Name
WISP1
Gene Alias
CCN4, WISP1c, WISP1i, WISP1tc
Gene Description
WNT1 inducible signaling pathway protein 1
Omim ID
603398Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
Other Designations
WNT1 induced secreted protein 1|Wnt-1 inducible signaling pathway protein 1|wnt-1 signaling pathway protein 1
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Interactomes
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Diseases
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Publication Reference
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Regulation of the Wnt signaling pathway by disabled-2 (Dab2).
Hocevar BA, Mou F, Rennolds JL, Morris SM, Cooper JA, Howe PH.
The EMBO Journal 2003 Jun; 22(12):3084.
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Human WISP1v, a member of the CCN family, is associated with invasive cholangiocarcinoma.
Tanaka S, Sugimachi K, Kameyama T, Maehara S, Shirabe K, Shimada M, Wands JR, Maehara Y.
Hepatology 2003 May; 37(5):1122.
Application:WB, Human, HuCCT1 cells.
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Overexpression of WISP-1 down-regulated motility and invasion of lung cancer cells through inhibition of Rac activation.
Soon LL, Yie TA, Shvarts A, Levine AJ, Su F, Tchou-Wong KM.
The Journal of Biological Chemistry 2003 Mar; 278(13):11465.
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Regulation of the Wnt signaling pathway by disabled-2 (Dab2).
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