NGF (Human) Recombinant Protein
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Specification
Product Description
Human NGF recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA with polyhistidine tag at the C-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 0.7 ng/mL, Measured by the induction of TF-1 cells proliferation. The specific activity of recombinant human beta-NGF is > 1 x 106 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of NGF (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NGF
Entrez GeneID
4803Gene Name
NGF
Gene Alias
Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Gene Description
nerve growth factor (beta polypeptide)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq
Other Designations
OTTHUMP00000013653|beta-nerve growth factor|nerve growth factor, beta polypeptide|nerve growth factor, beta subunit
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