Ngf monoclonal antibody, clone NGF30
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Specifications
Product Description
Rat monoclonal antibody raised against Ngf.
Immunogen
Mouse nerve growth factor (NGF).
Host
Rat
Reactivity
Mouse
Specificity
This antibody recognizes mouse NGF monomer protein (MW 13 KDa) by WB. It does not cross react with EGF, BSA, APO or insulin.
Form
Lyophilized
Isotype
IgG2a
Recommend Usage
Western Blot (1:10)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C or -20°C on dry atmosphere.
After reconstitution with water and glycerol (1:1), store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ngf
Entrez GeneID
18049Protein Accession#
P01138 (Human)Gene Name
Ngf
Gene Alias
Ngfb
Gene Description
nerve growth factor
Gene Ontology
HyperlinkGene Summary
beta
Other Designations
nerve growth factor, beta
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Publication Reference
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A nerve growth factor mimetic TrkA antagonist causes withdrawal of cortical cholinergic boutons in the adult rat.
Debeir T, Saragovi HU, Cuello AC.
PNAS 1999 Mar; 96(7):4067.
Application:Func, IHC, Rat, Rat cortex.
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A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals.
Saragovi HU, Zheng W, Maliartchouk S, DiGugliemo GM, Mawal YR, Kamen A, Woo SB, Cuello AC, Debeir T, Neet KE.
The Journal of Biological Chemistry 1998 Dec; 273(52):34933.
Application:Added, Recombinant protein.
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Two distinct monoclonal antibodies raised against mouse beta nerve growth factor. Generation of bi-specific anti-nerve growth factor anti-horseradish peroxidase antibodies for use in a homogeneous enzyme immunoassay.
Kenigsberg RL, Elliott PJ, Cuello AC.
Journal of Immunological Methods 1991 Feb; 136(2):247.
Application:C-ELISA, IF, IHC-Fr, WB-Re, Mouse, Mouse submandibular glands, PC-12 cells, Recombinant proteins, Septal cells.
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A nerve growth factor mimetic TrkA antagonist causes withdrawal of cortical cholinergic boutons in the adult rat.
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