ARF1 monoclonal antibody, clone AT1B3
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant ARF1.
Immunogen
Recombinant protein corresponding to full length human ARF1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry (2.5 ug for 106 cells)
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) HepG2 cell lysate, (3) brain tissue lysate and (4) MCF cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of MCF-7 cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Immunofluorescence
Immunofluorescent staining of U87 MG cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of ARF1 in MCF-7 cell line, staining at 2-5 ug for 1 x 106 cells (Red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (Black line). -
Gene Info — ARF1
Entrez GeneID
375Protein Accession#
NP_ 001649Gene Name
ARF1
Gene Alias
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Gene Description
ADP-ribosylation factor 1
Omim ID
103180Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000035715
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Publication Reference
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Arf1 coordinates fatty acid metabolism and mitochondrial homeostasis.
Ludovic Enkler, Viktoria Szentgyörgyi, Mirjam Pennauer, Cristina Prescianotto-Baschong, Isabelle Riezman, Aneta Wiesyk, Reut Ester Avraham, Martin Spiess, Einat Zalckvar, Roza Kucharczyk, Howard Riezman, Anne Spang.
Nature Cell Biology 2023 Aug; 25(8):1157.
Application:WB, Human, Yeast, HeLa cells, Saccharomyces cerevisiae.
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Arf1 coordinates fatty acid metabolism and mitochondrial homeostasis.
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