PPP1R14A (phospho T38) monoclonal antibody, clone AK-1F11
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More Files
- More Functions
Western Blot (Recombinant protein)
In vitro kinase reaction and detection of phosphorylation of CPI-17 at Thr38 residue by using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013). GST-CPI-17 was phosphorylated by protein kinase C derived from rat brain in vitro and proved by CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013) after SDS-PAGE and transfer to PVDF membrane.
Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant PKC activity using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of PPP1R14A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T38 of human PPP1R14A.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Phospho-CPI-17 Thr38 Antibody detects phosphorylated recombinant CPI-17 when phosphorylated at threonine38 in vitro by means of western blotting and ELISA.
Recommend Usage
Western Blot (1-2 ug/mL)
ELISA (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
In vitro kinase reaction and detection of phosphorylation of CPI-17 at Thr38 residue by using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013). GST-CPI-17 was phosphorylated by protein kinase C derived from rat brain in vitro and proved by CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013) after SDS-PAGE and transfer to PVDF membrane.Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant PKC activity using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013). -
Gene Info — PPP1R14A
Entrez GeneID
94274GeneBank Accession#
NM_033256Protein Accession#
NP_150281Gene Name
PPP1R14A
Gene Alias
CPI-17, CPI17, PPP1INL
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 14A
Omim ID
608153Gene Ontology
HyperlinkGene Summary
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
Other Designations
17-KDa protein|17-kDa PKC-potentiated inhibitory protein of PP1|PKC-potentiated inhibitory protein of PP1
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Interactome
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Pathway
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