CAMK4 monoclonal antibody (M01), clone 1A3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CAMK4.
Immunogen
CAMK4 (AAH25687.1, 1 a.a. ~ 473 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (79)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (77.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CAMK4 monoclonal antibody (M01), clone 1A3 Western Blot analysis of CAMK4 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody (M01), clone 1A3.
Lane 1: CAMK4 transfected lysate(52.14 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 ug/ml]ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi ( Cat # H00000814-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody (M01) clone 1A3 (Cat # H00000814-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CAMK4
Entrez GeneID
814GeneBank Accession#
BC025687Protein Accession#
AAH25687.1Gene Name
CAMK4
Gene Alias
CaMK-GR, MGC36771
Gene Description
calcium/calmodulin-dependent protein kinase IV
Omim ID
114080Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq
Other Designations
CAM kinase IV|CAM kinase- GR|brain Ca(2+)-calmodulin-dependent protein kinase type IV|brain Ca++-calmodulin-dependent protein kinase type IV|calcium/calmodulin-dependent protein kinase type IV catalytic chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Acid activation of Trpv1 leads to an up-regulation of calcitonin gene-related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade: a potential mechanism of inflammatory pain.
Nakanishi M, Hata K, Nagayama T, Sakurai T, Nishisho T, Wakabayashi H, Hiraga T, Ebisu S, Yoneda T.
Molecular Biology of the Cell 2010 Aug; 21(15):2568.
Application:IHC-P, Rat, Rat dorsal root ganglia.
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Acid activation of Trpv1 leads to an up-regulation of calcitonin gene-related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade: a potential mechanism of inflammatory pain.
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