RHOA monoclonal antibody (M08), clone 1C3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RHOA.
Immunogen
RHOA (AAH01360, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Host
Mouse
Reactivity
Human
Isotype
IgG1 Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.97 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RHOA monoclonal antibody (M08), clone 1C3 Western Blot analysis of RHOA expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RHOA
Entrez GeneID
387GeneBank Accession#
BC001360Protein Accession#
AAH01360Gene Name
RHOA
Gene Alias
ARH12, ARHA, RHO12, RHOH12
Gene Description
ras homolog gene family, member A
Omim ID
165390Gene Ontology
HyperlinkGene Summary
O
Other Designations
Aplysia ras-related homolog 12|oncogene RHO H12|small GTP binding protein RhoA
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Interactome
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Pathway
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Disease
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Publication Reference
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Effects of heterozygous deletion of autism-related gene Cullin-3 in mice.
Qiang-Qiang Xia, Angela K Walker, Chenghui Song, Jing Wang, Anju Singh, James A Mobley, Zhong X Xuan, Jeffrey D Singer, Craig M Powell.
PLoS One 2023 Jul; 18(7):e0283299.
Application:WB, Mouse, Mouse cortex, Mouse hippocampus.
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Effects of heterozygous deletion of autism-related gene Cullin-3 in mice.
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