AARS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AARS transfected lysate.
Western Blot
Lane 1: AARS transfected lysate ( 106.59 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AARS full-length
Host
Human
Theoretical MW (kDa)
106.59
Interspecies Antigen Sequence
Mouse (96); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AARS antibody (H00000016-B01) by Western Blots.
SDS-PAGE Gel
AARS transfected lysate.
Western Blot
Lane 1: AARS transfected lysate ( 106.59 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AARS
Entrez GeneID
16GeneBank Accession#
NM_001605.2Protein Accession#
NP_001596.2Gene Name
AARS
Gene Alias
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Gene Description
alanyl-tRNA synthetase
Omim ID
601065Gene Ontology
HyperlinkGene Summary
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq
Other Designations
alanine tRNA ligase 1, cytoplasmic
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Interactome
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Pathway
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