Peptide competition and stimulation extracts prepared from MO7e cells left untreated (lane 1) or treated with 100 ng/mL human SCF for 5 minutes (lane 2-4). The KIT (phospho Y721) polyclonal antibody (Cat # PAB10149) following prior incubation with : nopeptide (lane 1, 2), the non-phospho peptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphotyrosine-containing peptide (4).
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq