AKT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated or untreated with heat shock using AKT1 polyclonal antibody (Cat # PAB18076).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using AKT1 polyclonal antibody (Cat # PAB18076). Left : Untreated; Right: Treated with synthesized peptide.
Peptide "+" means "with peptide blocking".
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
Immunogen
A synthetic peptide corresponding to residues surrounding S473 of human AKT1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to AKT1.
Form
Liquid
Recommend Usage
Western Blot (1:100~1:500)
Immunohistochemistry (1:50~1:100)
ELISA(1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated or untreated with heat shock using AKT1 polyclonal antibody (Cat # PAB18076).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using AKT1 polyclonal antibody (Cat # PAB18076). Left : Untreated; Right: Treated with synthesized peptide.
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactomes
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Pathways
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Diseases
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Publication Reference
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High levels of phosphorylated form of Akt-1 in prostate cancer and non-neoplastic prostate tissues are strong predictors of biochemical recurrence.
Ayala G, Thompson T, Yang G, Frolov A, Li R, Scardino P, Ohori M, Wheeler T, Harper W.
Clinical Cancer Research 2004 Oct; 10(19):6572.
Application：IHC-P, Human, Human prostate cancer.
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Cyclosporin A inhibits flow-mediated activation of endothelial nitric-oxide synthase by altering cholesterol content in caveolae.
Lungu AO, Jin ZG, Yamawaki H, Tanimoto T, Wong C, Berk BC.
The Journal of Biological Chemistry 2004 Nov; 279(47):48794.
Application：WB, Bovine, Bovine aortic endothelial cells.
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Cytoplasmic mislocalization of p27Kip1 protein is associated with constitutive phosphorylation of Akt or protein kinase B and poor prognosis in acute myelogenous leukemia.
Min YH, Cheong JW, Kim JY, Eom JI, Lee ST, Hahn JS, Ko YW, Lee MH.
Cancer Research 2004 Aug; 64(15):5225.
Application：WB, Human, U-937 cells.
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Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation.
Feng J, Tamaskovic R, Yang Z, Brazil DP, Merlo A, Hess D, Hemmings BA.
The Journal of Biological Chemistry 2004 Aug; 279(34):35510.
Application：WB, Mouse, MEFs.
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S1P3-mediated Akt activation and cross-talk with platelet-derived growth factor receptor (PDGFR).
Baudhuin LM, Jiang Y, Zaslavsky A, Ishii I, Chun J, Xu Y.
FASEB Journal 2004 Feb; 18(2):341.
Application：WB, Human, Mouse, HEY cells, GI-101A cells, MEFs, T47D cells.
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High levels of phosphorylated form of Akt-1 in prostate cancer and non-neoplastic prostate tissues are strong predictors of biochemical recurrence.
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