PVR (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD96, mFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.21 ug/ml determined by ELISA.
Surface Plasmon Resonance
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
ELISA
Immobilized Human TIGIT, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1 ug/ml determined by ELISA (QC Test).
Tris-Bis PAGE
Human CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human PVR (P15151-1, Trp21-Asn343) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Trp21-Asn343
Host
Human
Theoretical MW (kDa)
61.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
ELISA
ELISA
Immobilized Human TIGIT, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1 ug/ml determined by ELISA (QC Test).
Tris-Bis PAGE
Human CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD96, mFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.21 ug/ml determined by ELISA.Functional Study
Surface Plasmon Resonance
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). -
Gene Info — PVR
Entrez GeneID
5817Protein Accession#
P15151-1Gene Name
PVR
Gene Alias
CD155, FLJ25946, HVED, NECL5, Necl-5, PVS, TAGE4
Gene Description
poliovirus receptor
Omim ID
173850Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
nectin-like 5
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Interactomes
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Pathways
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Diseases
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