


LILRB1 (Human) Recombinant Protein

* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Human LILRB1 (Q8NHL6-1, Gly24-Gly221) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Gly24-Gly221
Host
Human
Theoretical MW (kDa)
23.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
ELISA and SEC-HPLC
ELISA
Immobilized Human LILRB1 Domain1&2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LILRB1 Domain1&2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
SPR
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6 ng/mlFunctional Study
Surface Plasmon Resonance
Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200). -
Gene Info — LILRB1
Entrez GeneID
10859Protein Accession#
Q8NHL6-1Gene Name
LILRB1
Gene Alias
CD85, CD85J, FLJ37515, ILT2, LIR-1, LIR1, MIR-7, MIR7
Gene Description
leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Omim ID
604811Gene Ontology
HyperlinkGene Summary
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD85 antigen|Ig-like transcript 2|OTTHUMP00000068384|OTTHUMP00000068385|OTTHUMP00000068386|OTTHUMP00000068408|immunoglobulin-like transcript 2|leukocyte Ig-like receptor-1|leukocyte immunoglobulin-like receptor 1|leukocyte immunoglobulin-like receptor sub
-
Interactomes
-
Diseases
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com