

CLDN18 (Human) Recombinant Protein

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Specifications
Product Description
Human CLDN18 (P56856-2, Met1-Val261) partial recombinant protein expressed in HEK293 cells.
Sequence
Met1-Val261
Host
Human
Theoretical MW (kDa)
29
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
ELISA and SEC-HPLC
ELISA
Immobilized Human Claudin 18.2 VLP at 5 ug/ml (100 ul/Well). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (8% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Functional Study
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Gene Info — CLDN18
Entrez GeneID
51208Protein Accession#
P56856-2Gene Name
CLDN18
Gene Alias
SFTA5, SFTPJ
Gene Description
claudin 18
Omim ID
609210Gene Ontology
HyperlinkGene Summary
CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001 [PubMed 11585919]).[supplied by OMIM
Other Designations
surfactant associated 5|surfactant associated protein J|surfactant, pulmonary associated protein J
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