


CD276 (Human) Recombinant Protein

* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Human CD276 (Q5ZPR3-2, Leu29-Pro245) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Leu29-Pro245
Host
Human
Theoretical MW (kDa)
50.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human B7-H3, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.9 ng/ml determined by ELISA.Functional Study
-
Gene Info — CD276
Entrez GeneID
80381Protein Accession#
Q5ZPR3-2Gene Name
CD276
Gene Alias
B7-H3, B7H3
Gene Description
CD276 molecule
Omim ID
605715Gene Ontology
HyperlinkGene Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
Other Designations
B7 homolog 3|CD276 antigen
-
Interactomes
-
Pathways
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com