CD276 (Human) Recombinant Protein

Catalog # P10046

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Quantity

Size:100 ug
Price: USD $ 308.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

Immobilized Human B7-H3, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.9 ng/ml determined by ELISA.

SEC-HPLC
QC Test

SEC-HPLC

The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE
QC Test

Tris-Bis PAGE

Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

  • Specifications

    Product Description

    Human CD276 (Q5ZPR3-2, Leu29-Pro245) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro,CellTx,CellTherapy,Cell Therapy

    Sequence

    Leu29-Pro245

    Host

    Human

    Theoretical MW (kDa)

    50.1

    Form

    Lyophilized

    Preparation Method

    Mammalian cell (HEK293) expression system

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin Level

    < 1 EU per 1 ug of protein (determined by LAL method)

    Quality Control Testing

    SEC-HPLC

    SEC-HPLC

    The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.

    Tris-Bis PAGE

    Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

    Recommend Usage

    Biological Activity
    ELISA
    SEC-HPLC
    Tris-Bis PAGE
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).

    Storage Instruction

    After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Immobilized Human B7-H3, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.9 ng/ml determined by ELISA.

    Functional Study

  • Gene Info — CD276

    Entrez GeneID

    80381

    Protein Accession#

    Q5ZPR3-2

    Gene Name

    CD276

    Gene Alias

    B7-H3, B7H3

    Gene Description

    CD276 molecule

    Omim ID

    605715

    Gene Ontology

    Hyperlink

    Gene Summary

    Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM

    Other Designations

    B7 homolog 3|CD276 antigen

  • Interactomes
  • Pathways
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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