BTN3A1 (Human) Recombinant Protein
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Specifications
Product Description
Human BTN3A1 (O00481-1, Gln30-Gly254) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Gln30-Gly254
Host
Human
Theoretical MW (kDa)
25.3 kDa
Due to gForm
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human BTN3A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (8% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.Functional Study
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Gene Info — BTN3A1
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Interactomes
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Diseases
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