产品

Product Description

Human NT5E (P21589-1, Trp27-Lys547) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro,CellTx,CellTherapy,Cell Therapy

Sequence

Trp27-Lys547

Host

Human

Theoretical MW (kDa)

84.5 kDa
Due to g

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Quality Control Testing

ELISA, SEC-HPLC and Tris-Bis PAGE

ELISA

Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC₅₀ of 22.6ng/ml determined by ELISA.

SEC-HPLC

The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE

Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from filtered solution in 20 mM Tris, 120 mM NaCl, pH 7.5 (5% trehalose).

Storage Instruction

After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing.