VHH-His tag NanoAb™ Targeting Human GFAP
* The price is valid only in USA. Please select country.
- More Functions
Western Blot
Lysate of U87-MG cell line was subjected to SDS-PAGE followed by western blot with GFAP VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slides using GFAP VHH recombinant monoclonal antibody at 5ug/mL and positive control at 1:500 (under 20x lens), respectively. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde-fixed U251 cells stained with GFAP VHH recombinant monoclonal antibody at 10 ug/mL, showing cytoplasmic staining(under 40x lens).
-
Specifications
Product Description
VHH NanoAb™ targeting GFAP expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
13.2
Reactivity
SARS-CoV-2
Epitope
Monospecific
Tag
His tag at C-terminus
Specificity
Human Glial Fibrillary Acidic Protein (GFAP)
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography purified
Conjugation
Unconjugated
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
WB (1:1000-1:5000)
IHC (1:200-1:600)
IF (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Lysate of U87-MG cell line was subjected to SDS-PAGE followed by western blot with GFAP VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slides using GFAP VHH recombinant monoclonal antibody at 5ug/mL and positive control at 1:500 (under 20x lens), respectively. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde-fixed U251 cells stained with GFAP VHH recombinant monoclonal antibody at 10 ug/mL, showing cytoplasmic staining(under 40x lens).
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com