VHH-His tag NanoAb™ Targeting SARS-CoV-2 spike RBD
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- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein at 1 ug/mL (100 uL/well) can bind SARS-CoV-2 spike RBD VHH recombinant monoclonal antibody.
Surface Plasmon Resonance
Captured recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein on CM5 Chip can bind SARS-CoV-2 spike RBD VHH recombinant monoclonal antibody with an affinity constant of 6.6 nM as determined in SPR assay (Biacore T200).
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Specifications
Product Description
VHH NanoAb™ targeting SARS-CoV-2 Spike RBD expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
13.6
Reactivity
SARS-CoV-2
Epitope
Monospecific
Tag
His tag at C-terminus
Specificity
SARS-CoV-2 Spike RBD
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography purified
Conjugation
Unconjugated
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
ELISA (1:3000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein at 1 ug/mL (100 uL/well) can bind SARS-CoV-2 spike RBD VHH recombinant monoclonal antibody.Surface Plasmon Resonance
Captured recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein on CM5 Chip can bind SARS-CoV-2 spike RBD VHH recombinant monoclonal antibody with an affinity constant of 6.6 nM as determined in SPR assay (Biacore T200).
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