VHH NanoAb™ Targeting Human CD274 (iFluor 568)
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- More Functions
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed PD-L1 stable expressing PLC/PRF/5 cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at a dilution of 1:500 (under 40x lens). The nuclear stain is DAPI (blue).
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed SH-SY5Y cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at 0.7 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at 0.7 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
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Specifications
Product Description
VHH NanoAb™ targeting CD274 expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
14.4
Reactivity
Human
Epitope
Monospecific
Specificity
Human PD-L1
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography
Conjugation
iFluor 568
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
IF (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed PD-L1 stable expressing PLC/PRF/5 cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at a dilution of 1:500 (under 40x lens). The nuclear stain is DAPI (blue).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed SH-SY5Y cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at 0.7 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells stained with CD274 VHH recombinant monoclonal antibody (iFluor 568) at 0.7 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
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