VHH NanoAb™ Targeting GFP (iFluor 488)
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Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed GFP-expressing Neuro-2a cells stained with GFP VHH recombinant monoclonal antibody (iFluor 488) at 0.2 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed GFP-expressing SH-SY5Y cells stained with GFP VHH recombinant monoclonal antibody (iFluor 488) at 0.2 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
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Specifications
Product Description
VHH NanoAb™ targeting GFP expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
13.7
Reactivity
Jellyfishes
Epitope
Monospecific
Specificity
GFP and other derivatives of GFP
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography
Conjugation
iFluor 488
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
IF (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed GFP-expressing Neuro-2a cells stained with GFP VHH recombinant monoclonal antibody (iFluor 488) at 0.2 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed GFP-expressing SH-SY5Y cells stained with GFP VHH recombinant monoclonal antibody (iFluor 488) at 0.2 ug/mL (under 60x lens). The nuclear stain is DAPI (blue).
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