Fluorescence In Situ Hybridization (FISH)

FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. Abnova has developed a range of FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library. We continue to expand the scope of the FISH probes to meet the customer’s research needs. Custom probes are also available upon request.

Advantages

Dual colored probes for fast, sensitive, and specific detection
Work on metaphase spread, paraffin embedded and frozen tissue
Identify gene amplification, loss, and translocation
High signal to noise ratio
Low cross reactivity

Product Lines

Gene Amplification / Gene Loss

Split Dual Color FISH Probe

Translocation Dual Color FISH Probe

Procedure

Publication Reference

DNA sequence and analysis of human chromosome 8
Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, and et al. Nature. 2006 Jan 19;439(7074):331-5.

Initial sequencing and analysis of the human genome
Lander ES, Linton LM, Birren B, Nusbaum C, and et al. Nature. 2001 Feb 15;409(6822):860-921.

A physical map of the human genome.
McPherson JD, Marra M, Hillier L, Waterston RH, and et al. Nature. 2001 Feb 15;409(6822):934-41.

Human BAC library: construction and rapid screening.
Asakawa S, Abe I, Kudoh Y, Kishi N, Wang Y, Kubota R, Kudoh J, Kawasaki K, Minoshima S, Shimizu N. Gene. 1997 May 20;191(1):69-79.