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Pseudoviruses are useful virological tools because of versatility and safety for emerging (SARS-CoVs-2) and re-emerging viruses (MERS-CoV, Nipah virus). They can be generated and handled in a biosafety level 2 facility, unlike live SARS-CoV-2 virus under a biosafety 3 level condition. Using the Coronavirus spike lentiviral vector system with luciferase (Luc) reporter gene, different pseudoviruses can be generated with different outer membrane proteins essential for viral entry into a surface receptor expressing 293T cells. Specifically, a combination of SARS-COV-2 pseudovirus with spike (S) protein and 293T cell expressing angiotensin-converting enzyme 2 (ACE2) receptor protein will be valuable platform for evaluating neutralizing antibodies and viral entry inhibitors against this highly pathogenic virus.

Pseudovirus Production

Pseudovirus Advantages

  • Versatile with different outer membrane proteins
  • Safe without virulent viral components
  • Compatible with biosafety level 2 facility
  • Non-labor intensive
Target Cell Line Transfection

Antibody Neutralization

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