mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)

Single Cell, Single Molecule, DNA and RNA Mutation Detection at Single Nucleotide Resolution

The ability to interrogate cellular heterogeneity at the single cell and single molecule levels has gained increased importance. Many bioreagents and techniques exist to address this issue separately. None has the unified approach to detect and quantify DNA and RNA changes across diverse spectrum particularly point mutations and single-nucleotide polymorphisms (SNP) while maintaining their spatial context. This challenge is even greater as different analytical platforms such fluorescence, cyto/histochemistry, flow cytometry, and microfluidics are needed to study the complexity of biological states.

Abnova has integrated padlock probe and rolling circle amplification (RCA) for in situ, single cell, single molecule, DNA and RNA mutation detection at single nucleotide resolution, and obviated the technical challenges of PCR and hybridization optimization required for efficient and target-specific analysis. Abnova provides a growing portfolio of off-the-shelf, validated mutaFISH™ probes and accessory reagents to address the unmet needs in the research and clinical settings.

Download mutaFISH™

mutaFISH™ Workflow

mutaFISH™ - How it works

Advantage

Single Cell, Single Molecule Detection
In Situ Analysis of DNA & RNA
Single Nucleotide Resolution
Higher Sensitivity than dPCR and NGS
Multiplexing Capability
Cross Analytical Platforms
No DNA or RNA Extraction

Applications

Cell In Situ Hybridization

Circulating Tumor Cell In Situ Hybridization

Fresh Frozen Tissue In Situ Hybridization

Formalin-Fixed Paraffin-Embedded Tissue In Situ Hybridization

Flow Cytometry Mutation-Based Cell Sorting

Microfluidic Cell Isolation & In Situ Hybridization

mutaFISH™ Probes vs Conventional DNA and RNA FISH Probes

	mutaFISH™ Probe	Conventional DNA FISH Probe	Conventional RNA FISH Probe
Point Mutation	Yes	No	No
Gene Amplification	Yes	Yes	No
Gene Deletion	Yes	Yes	No
Gene Translocation	Yes	Yes	No
Gene Expression	Yes	No	Yes

Publication Reference

In situ mutation detection and visualization of intratumor heterogeneity for cancer research and diagnostics.
Grundberg I, Kiflemariam S, Mignardi M, et al. Oncotarget. 2013;4(12):2407-18.

In situ detection and genotyping of individual mRNA molecules.
Larsson C, Grundberg I, Söderberg O, Nilsson M. Nat Methods. 2010;7(5):395-7.

Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification.
Nilsson M. Histochem Cell Biol. 2006;126(2):159-64.

In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.
Larsson C, Koch J, Nygren A, et al. Nat Methods. 2004;1(3):227-32.