Rabbit Monoclonal Antibody (Phage Antibody Library)

Integrated, High Throughput Antibody Production

Rabbit monoclonal antibody (Mab) is secreted by the plasma B-cell of the rabbit. Traditional generation of rabbit Mab relies on a rabbit myeloma for B-cell fusion (<1% fusion rate) and hybridoma development. Rabbit Monoclonal Antibody (Phage Antibody Library, PAL) uses a non-fusion Fab antibody library to produce rabbit Mab in vitro. PAL platform utilizes the entire rabbit antibody repertoire to screen for high affinity and high specificity Fab binders with broad epitope coverage to antigen of interest. PAL has higher antibody screening throughput and success rate. PAL Fab is amenable to antibody engineering including F(ab)2, IgG, scFv and multitude of different Fc and non-Fc conjugates. PAL technology is the solution to high quality reagents for research and commercial developments.

Rabbit Monoclonal Production Workflow

Abnova Rabbit Mab (PAL) vs. Conventional Rabbit Mab

	Abnova Rabbit Mab (PAL)	Conventional Rabbit Mab
Technology	Non-Fusion Fab Antibody Library	Rabbit Myeloma
Antibody Repertoire	Broad (no fusion needed)	Narrow (<1% fusion rate)
Screening Throughput	High	Low
Epitope Coverage	High	Low
Antibody Success Rate	Higher	Lower
Antibody Yield	High	Low
Clone Stability	Excellent	Poor
Antibody Engineering	Readily Applicable	Troublesome

Rabbit vs. Mouse Mab

	Rabbit	Mouse
Immune Tolerance	None	Yes
Antibody Diversity	Broad	Narrow
Antigenicity	Strong reaction to small molecules,peptides, & proteins.	Weak reaction to small molecules and peptides.
Affinity	Picomolar	Nanomolar
Specificity	High	Moderate
Isotype	IgG	IgG1, IgG2a, IgG2b, IgG3
Applications	Especially suited for IHC, IP, FC, antibody pair & kit.	Mostly suited for WB, ELISA, IF, antibody pair & kit.
Antibody Engineering	Yes	Yes


Advantages	Applications

High Affinity Antibody Diverse Antibody Repertoire No Immunologic Tolerance Scalable Antibody Production More Stable Antibody Clone Antibody Engineering	High Affinity Antibody Rabbit Monoclonal Antibody Modification-State Antibody Anti-Idiotype Antibody Antibody Pairing and ELISA Kit Research and In Vitro Diagnostic

For any inquiry, please contact : OEM@abnova.com