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Humanized Ab

Abnova’s humanized antibody service starts with mouse monoclonal antibody production via mouse immunization, splenic fusion, hybridoma screening, antibody selection, and isotype determination. The selected mouse monoclonal antibody undergoes variable gene sequencing for complementarity-determining region (CDR) grafting, and if needed specificity determining residues (SDR) grating, onto the human antibody construct followed by sequence alignment and production. The humanized antibody is then characterized by a series of in vitro and in vivo assays to assure its biochemical, biophysical, and functional properties. Moreover, the Fc region of the humanized antibody is mutated to minimize antibody-dependent enhancement (ADE) immune response.

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Monoclonal Antibody Production

  • Antigen Preparation
  • Mouse Immunization
  • Hybridoma Screening
  • Antibody Selection
  • Isotype Determination
Monoclonal Antibody Humanization

  • Mouse Antibody Variable Gene Sequencing
  • Complementarity-Determining Region (CDR) Grafting
  • Specificity Determining Residue (SDR) Grating
  • Humanized Antibody Sequence Alignment
  • Humanized Antibody Variant Production

Humanized Antibody Characterizations

  • Protein and Peptide Mapping
  • Affinity Kinetic Assay
  • Binding, Inhibition, Neutralization Assays
  • Cytokine, ADCC/CDC, and Toxicology Assays
  • Animal Assay
Humanized Antibody Fc Engineering

  • LALAPG mutation
  • LALANG mutation


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