Mouse, Rat & Rabbit Polyclonal Ab (All)

Abnova has extensive experience in generating high quality antisera to a wide variety of antigens (peptides, phospho-peptides, proteins, and DNAs). Using a low dose immunization technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against the immunogen in mice, rats, and rabbits.

Polyclonal Antibody Development Workflow

Evaluation of Standard Antibody Production Service
Pre-Production Discussion: Custom Specification, Flowchart, Cost
Purchase Order
Production Specification Approval
Production Specification Template
Polyclonal Antibody Development and Progress Report
Polysera Delivery

Mouse & Rat

Download Custom Pab Service

Service Items	Time (weeks)	Antigen Supplied by Customer			Antigen Supplied by Abnova
					Peptide		Phospho-peptide	Protein		DNA
Peptide Design & Synthesis
Customer-supplied antigen	4 ~ 5	V	V	V
7~20-mer (Purity > 80%)					V	V	V
Single site phosphorylation (Ser, Tyr, Thr)							V
Conjugation
To carrier protein KLH/BSA/OVA (A cysteine is automatically added to the C-terminus or N-terminus of the peptide)	1				V		V
Protein Preparation
DNA sourcing	2~3							V	V
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing	2~3									V	V
Cloning and sequencing
Immunization
5~8 injections per animal (3 mice or 3rats)	8 ~ 12	V	V	V	V	V	V	V	V	V	V
Sera collection
Report
ELISA data *	1	V	V	V	V	V	V	V	V	V	V
WB data (mammalian overexpression lysate)										V	V
Purification **
By protein A purification Final deliverable: > 0.2 mg purified antibody for mice or > 5 mg purified antibody for rats	2		V		V			V		V
By affinity purification (for rats only) Final deliverable: > 0.5 mg purified antibody				V		V	V		V		V
Total Lead time (weeks)		10~12	12~14	12~14	17~20	17~20	17~20	19~20	19~20	12~16	12~16
Total Price (USD/EUR)		Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote

Rabbit

Service Items	Time (weeks)	Antigen Supplied by Customer			Antigen Supplied by Abnova
					Peptide		Phospho-peptide	Protein		DNA
Peptide Design & Synthesis
Customer-supplied antigen	4 ~ 5	V	V	V
7~20-mer (Purity > 80%)					V	V	V
Single site phosphorylation (Ser, Tyr, Thr)							V
Conjugation
To carrier protein KLH/BSA/OVA (A cysteine is automatically added to the C-terminus or N-terminus of the peptide)	1				V	V	V
Protein Preparation
DNA sourcing	2~3							V	V
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing	2~3									V	V
Cloning and sequencing
Immunization
5~8 injections per rabbit (2 rabbits)	10 ~ 12	V	V	V	V	V	V	V	V	V	V
Sera collection
Report
ELISA data *	1	V	V	V	V	V	V	V	V	V	V
WB data (mammalian overexpression lysate)										V	V
Purification **
By protein A purification Final deliverable: 20-50 mg purified antibody	2		V		V			V		V
By affinity purification Final deliverable: 1-5 mg purified antibody				V		V	V		V		V
Total Lead time (weeks)		11~14	13~16	13~16	17~20	17~20	17~20	19~20	19~20	12~16	12~16
Total Price (USD/EUR)		Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote

Note:
* Polyclonal antibody sera will be QC utilizing ELISA format for reactivity against the immunogen.
** This is the average yield. Actual yield will vary for each Pab.

The above list price is only used for direct orders made to Abnova.

For any inquiry, please contact : OEM@abnova.com