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Camelid single domain antibody (SdAb), also known as VHH and nanobody, is the recombinant variable domain derived from camelid heavy-chain-only antibody. This configuration confers a number of unique properties. The small size and high stability of SdAb make it a promising tool for disease diagnosis and treatment. SdAb can penetrate tissue more easily and target difficult epitopes hidden in protein structure. Also, SdAb expresses well in many host systems and it is cost-effective to manufacture on a large scale.

Abnova offers Camelid Single Domain Antibody Service for production of target-specific SdAb from naïve camelid VHH library. By using the advanced naïve camelid VHH library screening technology, we screen the library and isolate the SdAb clones for your required antigens. Positive clones are sequenced and validated by ELISA. The selected clones will then be introduced into HEK293 expression vector or E. coli expression vector for transient and scalable SdAb production for many downstream applications.

Related FAQ
Naïve Camelid VHH Library Screening Workflow

  • SdAb clone isolated from naive Camelid VHH library
  • Library size: 1.2 x 1012
  • Required screening antigen amount : 600μg
  • Lead Time: 8-10 weeks
  • Deliverables:
     1. Phage validation by phage ELISA data
     2. Up to 5 different VHH sequences
     3. Up to 5 purified SdAbs
Advantages of Camelid SdAb

  • High Affinity Small Size Antibody: Small size around 12-15 kD with high affinity and specificity
  • High Stability: Stable in a wide range of temperatures and at extreme pH levels
  • Enhanced Tissue Penetration: Better tissue penetration than IgG due to their small size
  • Small Drug Feature: Recognize hidden antigenic sites in harsher conditions (heat, pH)
  • Effective Manufacturing: Express well in various platforms, formulated at high concentrations, easy to be scaled up
  • Animal Protection: No animal immunization
  • High Diversity Libraries: Naïve VHH library size of 1.2 x 1012
Production of Camelid SdAb vs. Conventional Mab and Pab

  Camelid SdAb Mab Pab
 Size 12 - 15 kD Approximately 150 kD Approximately 150 kD
 Immunization No animal immunization Animal immunization required Animal immunization required
 Required Antigen Amount ≦1 mg ≦4 mg ≦4 mg
 Method Naïve camelid VHH library screening Hybridoma technology Harvested in antiserum
 Stability High stability to heat, pH and denaturing agents Thermal instability Biological instability
 Lot-to-Lot Consistency Very high High Low
 Lead Time 8-10 weeks 16-24 weeks 4-12 weeks

ELISA Results of VHH Clone Isolation
ELISA results of selected phage clones after 6 rounds of panning
ELISA Results of Selected Clones as Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab
ELISA QC of selected clones Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab

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