Nanobody

Camelid single domain antibody (SdAb), also known as VHH and nanobody, is the recombinant variable domain derived from camelid heavy-chain-only antibody. This configuration confers a number of unique properties. The small size and high stability of SdAb make it a promising tool for disease diagnosis and treatment. SdAb can penetrate tissue more easily and target difficult epitopes hidden in protein structure. Also, SdAb expresses well in many host systems and it is cost-effective to manufacture on a large scale.

Abnova offers Camelid Single Domain Antibody Service for production of target-specific SdAb from naïve camelid VHH library. By using the advanced naïve camelid VHH library screening technology, we screen the library and isolate the SdAb clones for your required antigens. Positive clones are sequenced and validated by ELISA. The selected clones will then be introduced into HEK293 expression vector or E. coli expression vector for transient and scalable SdAb production for many downstream applications.

Related FAQ

Naïve Camelid VHH Library Screening Workflow

SdAb clone isolated from naive Camelid VHH library
Library size: 1.2 x 10¹²
Required screening antigen amount : 600μg
Lead Time: 8-10 weeks
Deliverables:
1. Phage validation by phage ELISA data
2. Up to 5 different VHH sequences
3. Up to 5 purified SdAbs

Advantages of Camelid SdAb

High Affinity Small Size Antibody: Small size around 12-15 kD with high affinity and specificity
High Stability: Stable in a wide range of temperatures and at extreme pH levels
Enhanced Tissue Penetration: Better tissue penetration than IgG due to their small size
Small Drug Feature: Recognize hidden antigenic sites in harsher conditions (heat, pH)
Effective Manufacturing: Express well in various platforms, formulated at high concentrations, easy to be scaled up
Animal Protection: No animal immunization
High Diversity Libraries: Naïve VHH library size of 1.2 x 10¹²

Production of Camelid SdAb vs. Conventional Mab and Pab

	Camelid SdAb	Mab	Pab
Size	12 - 15 kD	Approximately 150 kD	Approximately 150 kD
Immunization	No animal immunization	Animal immunization required	Animal immunization required
Required Antigen Amount	≦1 mg	≦4 mg	≦4 mg
Method	Naïve camelid VHH library screening	Hybridoma technology	Harvested in antiserum
Stability	High stability to heat, pH and denaturing agents	Thermal instability	Biological instability
Lot-to-Lot Consistency	Very high	High	Low
Lead Time	8-10 weeks	16-24 weeks	4-12 weeks

Examples

ELISA Results of VHH Clone Isolation

ELISA results of selected phage clones after 6 rounds of panning

ELISA Results of Selected Clones as Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab

ELISA QC of selected clones Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab

For any inquiry, please contact : OEM@abnova.com