PCR : we have 12 products

10 x Taq Buffer is a ready-to-use buffer to be used for Taq, Hot Taq, Red Taq and Long Taq enzymes in PCR reactions. Does not contain MgCl₂.

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10 x Taq Buffer with (NH₄)₂SO₄ is a ready-to-use buffer to be used for Taq, Hot Taq, Red Taq and Long Taq enzymes in PCR reactions. Does not contain MgCl₂.

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HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes highquality recombinant Taq DNA polymerase, nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set; total volume of 50 ul). PCR Mix is provided with MgCl₂ in the solution.

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2 x RedTaq PCR Mix with MgCl2 is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes highquality recombinant LongTaq DNA polymerase, nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set; total volume of 50 ul). PCR Mix is provided with MgCl₂ in the solution.

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The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template and water for the total reaction volume of 20 or 50 uL.

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25 mM MgCl₂ can be used for optimization of magnesium ion concentration in PCR reaction.

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HotTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. Prior the first PCR step the HotTaq DNA polymerase should be activated by 15 minute incubation at 95-97°C.

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LongTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure.

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Manufactured and tested for use as a component in PCR reactions. Validated as well for use with highly sensitive PCR reaction including those with eubacterial primer sets. Stored in convenient 2 mL tubes to avoid unnecessary melting and freezing of the whole water supply.

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In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

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RedTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure. Added inert dye will not have any interference to the reaction. Visual confirmation that the enzyme has been added and proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition.

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Taq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure.

• • PCR