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Last updated: 2023/3/26

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SARS-CoV-2 Pseudovirus Expressing Luciferase Coronavirus

  • Catalog # : U0401
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  • Specification
  • Product Description:
  • SARS-CoV-2 Pseudovirus Expressing Luciferase (wild type) can be used to measure neutralizing antibody against SARS-CoV-2.
  • Activity:
  • 1.2x106 TU/mL
    The titer of lentiviral particles is given as transduction units (TU) per milliliter. 1TU = 1 infectious particle.
  • Storage Instruction:
  • Store at -80°C
    Aliquot to avoid repeated freezing and thawing.
  • Materials Required But NOT Supplied:
  • 1. 293T-hACE2 cell
    2. SARS-CoV-2 Monoclonal Antibody, Cat# MAB22854-M07, Abnova
    3. ZymeFree™ Enzyme Free Cell Dissociation Reagent,
     Cat# TCL028-100ML, HIMEDIA
    4. DMEM culture medium containing 10% FBS (complete medium)
    5. Luciferase Assay System, Cat# E1500, Promega
     a. Luciferase Assay Substrate (lyophilized)
     b. Luciferase Assay Buffer
     c. Luciferase Cell Culture Lysis Reagent, 5X
    6. TC-Treated 24 Well Plates, Cat# 3524, Corning
    7. OptiPlate™-96, Cat# 6005290, PerkinElmer
  • Suitable Sample:
  • 1. Immunized Serum
    2. Infected Serum
    3. Neutralizing Antibodies
    4. Peptide Inhibitors or Proteins
    5. Compounds Targeting Spike Induced Cell Fusion
  • Sample Volume:
  • 10-50 uL (please check sample source)
  • Detection Method:
  • Luciferase Activity
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Neutralization
  • Neutralization
  • RBD-binding IgG responses in mouse sera collected 35 days and 42 days after immunization with 5 ug or 20 ug COVID-19 RBD SAM LNP no adjuvant (ppopV0) and 5 ug or 20 ug COVID-19 RBD SAM plus adjuvant (ppopV1).
  • Neutralization
  • Neutralization
  • SARS-CoV-2 spike pseudovirus expressing luciferase is treated with humanized monoclonal antibody 7F7 and LALAPG 7F7, and then added into 293T-ACE cells.
  • In vivo Neutralization
  • <i>In vivo</i> Neutralization
  • Hamsters received 2 doses of humanized monoclonal antibody 7F7 and then were infected with SARS-CoV-2 Pseudovirus Expressing Luciferase (Cat # U0401).
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