ALFA Selector ST is based on a single-domain antibody (sdAb) that is for the highly efficient pull-down of ALFA-tagged target proteins.
Host:
Camelid
Specificity:
Recognizes ALFA-tagged proteins.
Form:
Liquid
Recommend Usage:
ChIP Co-Immunoprecipitation Immunoprecipitation The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (20% ethanol, 50% slurry).
Storage Instruction:
Store at 4°C. Do not freeze.
Note:
ALFA Selector ST is covalently immobilized on 4% cross-linked agarose beads and binds ALFA-tagged target proteins with extraordinarily high affinity. Target proteins are efficiently released from ALFA Selector ST under acidic or denaturing conditions. Due to the extremely tight interaction with ALFA-tagged proteins, competitive elution using ALFA elution peptide is inefficient. ALFA Selector ST is compatible not only with physiological buffers but also with high stringency buffers. It thus provides great freedom to adjust the binding and washing conditions to the experimental needs. It is selected from the alpaca in vitro synthetic gene library, produced by phage display derived recombinant antibodies.