FluoTag-Q anti-GFP single-domain antibody (sdAb, also known as a nanobody) raised against GFP and its most common derivatives.
Host:
Camelid
Specificity:
Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Form:
Lyophilized
Conjugation:
AZDye 568 Each fluorophore of the FluoTag-Q series is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion.
Concentration:
5 uM
Recommend Usage:
Immunofluorescence (1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS pH 7.4 containing 5 uM fluorescently labeled sdAb. Reconsitute with 200 uL 50% glycerol.
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Note:
It is selected from the alpaca in vitro synthetic gene library, produced by phage display derived recombinant antibodies.