MAPK8 (phospho T183) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPK8.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T183 of human MAPK8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of MAPK8 (phospho T183) polyclonal antibody (Cat # PAB8112) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — MAPK8
Entrez GeneID
5599Protein Accession#
P45983Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
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Interactome
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Publication Reference
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A JNK-dependent pathway is required for TNFalpha-induced apoptosis.
Deng Y, Ren X, Yang L, Lin Y, Wu X.
Cell 2003 Oct; 115(1):61.
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Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase.
Ghosh J.
Biochemical and Biophysical Research Communications 2003 Jul; 307(2):342.
Application:WB-Ce, Human, LNCaP cells.
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Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation.
Luo W, Ng WW, Jin LH, Ye Z, Han J, Lin SC.
The Journal of Biological Chemistry 2003 Jul; 278(39):37451.
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A JNK-dependent pathway is required for TNFalpha-induced apoptosis.
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