RHOA (phospho S188) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RHOA.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S188 of human RHOA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RHOA
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Interactome
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Pathway
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Disease
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Publication Reference
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Serine phosphorylation negatively regulates RhoA in vivo.
Ellerbroek SM, Wennerberg K, Burridge K.
The Journal of Biological Chemistry 2003 May; 278(21):19023.
Application:IP, WB-Tr, Mouse, NIH 3T3 cells.
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cGMP-dependent protein kinase phosphorylates and inactivates RhoA.
Sawada N, Itoh H, Yamashita J, Doi K, Inoue M, Masatsugu K, Fukunaga Y, Sakaguchi S, Sone M, Yamahara K, Yurugi T, Nakao K.
Biochemical and Biophysical Research Communications 2001 Jan; 280(3):798.
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cAMP-induced morphological changes are counteracted by the activated RhoA small GTPase and the Rho kinase ROKalpha.
Dong JM, Leung T, Manser E, Lim L.
The Journal of Biological Chemistry 1998 Aug; 273(35):22554.
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Serine phosphorylation negatively regulates RhoA in vivo.
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