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Last updated: 2023/5/29
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VPS35 polyclonal antibody 

  • Catalog # : PAB7499
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of VPS35.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human VPS35.
  • Sequence:
  • C-SPESEGPIYEGLIL
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 91
  • Reactivity:
  • Human
  • Specificity:
  • There is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Immunohistochemistry (8 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • VPS35 polyclonal antibody (Cat # PAB7499) staining (1 ug/mL) of human cerebellum lysate (RIPA buffer, 35 ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • VPS35 polyclonal antibody (Cat # PAB7499) (8 ug/mL) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • VPS35 polyclonal antibody (Cat # PAB7499) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Immunofluorescence
  • Immunofluorescence
  • VPS35 polyclonal antibody (Cat # PAB7499) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • VPS35
  • Gene Alias:
  • DKFZp434E1211,DKFZp434P1672,FLJ10752,FLJ13588,FLJ20388,MEM3
  • Gene Description:
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • Gene Summary:
  • This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
  • Other Designations:
  • maternal-embryonic 3,vacuolar protein sorting 35
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