AKR1B10 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
AKR1B10 polyclonal antibody (Cat # PAB7448) (0.03 ug/mL) staining of A-549 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AKR1B10.
Immunogen
A synthetic peptide corresponding to human AKR1B10.
Sequence
C-QSSHLEDYPFDAE
Host
Goat
Theoretical MW (kDa)
36
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
AKR1B10 polyclonal antibody (Cat # PAB7448) (0.03 ug/mL) staining of A-549 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKR1B10
Entrez GeneID
57016Protein Accession#
NP_064695.2Gene Name
AKR1B10
Gene Alias
AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI, MGC14103
Gene Description
aldo-keto reductase family 1, member B10 (aldose reductase)
Omim ID
604707Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq
Other Designations
aldo-keto reductase family 1, member B10|aldo-keto reductase family 1, member B11 (aldose reductase-like)|aldose reductase-like 1|aldose reductase-like peptide|aldose reductase-related protein|small intestine reductase
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Interactome
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Pathway
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Disease
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Publication Reference
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Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells.
Tammali R, Ramana KV, Singhal SS, Awasthi S, Srivastava SK.
Cancer Research 2006 Oct; 66(19):9705.
Application：WB-Tr, Human, Caco-2 cells.
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Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells.
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